"(evil laugh) You were right! I didn't understand magic before... but I do now!!!" - Midnight speaking to The Rainbooms
Midnight Sparkle (voiced by Tara Strong) is Sci-Twi's alter ego and Princess Twilight's dark side who first introduced as the ultimate antagonist in Twilight and Kyle's Adventures of Equestria Girls: Friendship Games.
- Although Sunset Shimmer saved Sci-Twi from her alter ego, it is known her dark side somehow got infected on her Equestrian counterpart, which her dark side is named as "Midnight Sparkle".
- Midnight will become Jeffrey and Jaden's enemy in the future crossovers whenever Twilight loses control as the darkness spreads all over her.
- Midnight will return in Twilight's Adventures of Equestria Girls: Legend of Everfree, and after that, she will replace Adagio Dazzle as the main antagonist of the Twilight's Adventures series.
- In the Thunderbolts Adventure Series, Midnight Sparkle is haunting Courtney Elison and vows to get her power at all costs.