Dr. Nefarious (voiced by Armin Shimerman) is the main antagonist of Ratchet and Clank series, and serves as Anubis' evil right-hand man, just like The Chameleon serves as Set's right-hand man. He is a robotic scientist who hated organic life forms, despite formerly being one himself. He was the archenemy of so-called "super hero" Captain Qwark, and later recurring enemy of Ratchet and Clank. Anubis promised him that he will kill three of them if he helps Anubis to destroy Twilight and her friends by stealing their Equestrian magic and annihilate them.
- Dr. Nefarious will become Jeffrey and Jaden's enemy in the near future as The Justice Guardians teams up with The Rainbooms.