
Doctor Octopus, real name Otto Gunther Octavius, (voiced by the late Efrem Zimbalist Jr., Peter MacNicol, Tom Kenny, Scott Menville, and William Salyers) is a major antagonist in the Marvel Comics, serving as the secondary antagonist towards Spider-Man, and one of the secondary antagonists of Twilight's Adventures Series a.k.a. "Twilight Sparkle and Friends"/"The Rainbooms". He is the founder of Sinister Six.
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- Doc Ock will become Twilight's enemy in Twilight Sparkle meets The Spectacular Spider-Man, and at the end, he joins the Heylin side to serve Anubis.
- Doc Ock will return for revenge in Twilight's Adventures of Spider-Man: The Animated Series. From then on, he'll become one of Twilight and her team's most recurring and dangerous foes.
- Despite being ruthless and affiliated with the Heylin side with no remorse of his actions, Otto is one of the few enemies who reminds Twilight of his live-action counterpart she first met, and feels a very little sympathy for him as well sometimes.
- Doc Ock will return in Twilight's Adventures of Batman & Mr. Freeze: SubZero.
- Doc Ock will appear in a flashback fighting Twilight, Peter and the Rainbooms in Jeffrey, Jaden, Hiccup, Twilight, Courtney & Craig's Storm Adventures of Spider-Man: Into the Spider-Verse.