B.O.B. a.k.a. Benzoate Ostylezene Bicarbonate (Vocied by Seth Rogen) is a blue one-eyed indestructible gelantinous mass that was created in a laboratory when scientists injected a chemically-altered ranch dressing into a genetically-altered tomato in Monsters vs. Aliens. As explained by Dr. Cockroach, B.O.B has no brain.
- Jeffrey will meet B.O.B. in Jeffrey & Friends' Adventures of Monsters vs. Aliens.
- B.O.B. will attend Jeffrey and Aqua's wedding in Key of Flaming Union.
- B.O.B will guest star in Jeffrey, Jaden & Friends' Storm Adventures of Stitch!: The Movie.